Analytical Ultracentrifugation of Polymers and Nanoparticles by Walter Maechtle

By Walter Maechtle

This booklet is split into chapters protecting instrumentation, sedimentation pace runs, density gradient runs, software examples and destiny advancements. specifically, the unique program bankruptcy demonstrates the flexibility and tool of AUC via many fascinating and critical business examples. hence the e-book concentrates on functional points instead of information of centrifugation idea.

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Extra resources for Analytical Ultracentrifugation of Polymers and Nanoparticles (Springer Laboratory)

Example text

11] inside a Model E (reprinted with permission from [11]) 40 2 Analytical Ultracentrifugation, Instrumentation reflectance [12] or in transmission mode [11] by a PM tube. A filter is used in front of the photomultiplier to ensure that only fluorescence light passes and excitation light is omitted. Riesner et al. described the first fluorescence detector adapted to an AUC [11]. They used the geometry of the Schlieren optics channel of a Model E (see Fig. 11), and replaced the Model E components of the Schlieren optics by the components of the fluorescence detector.

Even steepest refractive index gradients (resulting often from steep density gradients) can be followed because these gradients can be compensated via wedge windows (as will be shown in Chap. 4, density gradients are among the most important experiments in AUC). The modification of the preparative Optima XL centrifuge itself, especially the addition of a Schlieren optical system, has been outlined in Sect. 2. In this paragraph, only the necessary optical components will therefore be described.

4: the determination of particle densities in AUC density gradients. The use of a pycnometer is also possible, but tedious. Furthermore, a classical method to determine solution densities is the use of calibrated sinkers/floaters that make use of the Archimedes principle. Edelstein and Schachman [24] determined for special cases the partial specific volume by variation of solvents. They measured the product M(1 − ν¯ · ρs ), called the effective molar mass, in different solvents with varying known densities, such as H2 O and D2 O, using equilibrium runs (see Chap.

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